(Title image: Ultrasound-encoded wavefront used for optical resolution imaging with diffuse light. See publications)
We are a group of neuroscientists, engineers, biologists and physicists who develop and apply new optical techniques to study brain circuits at depths that have long been unreachable. The lab is part of the Einstein Center for Neurosciences and is located on the Charité / Humboldt University campus in the centre of Berlin.
Benjamin Judkewitz, professor in Bioimaging and Neurophotonics.
2010 - 2014: Sir Henry Wellcome Postdoctoral fellow at the California Institute of Technology, departments of Electrical Engineering and Bioengineering
2006 - 2010: PhD in Neuroscience and Physiology at University College London.
2000- 2005: Biology undergraduate training in Heidelberg, Berkeley and at the Max Planck Institute for Medical Research.
If all our bodies were as transparent as these jellyfish, the implications for biomedical science would be tremendous. Biologists could directly look at deep tissues to study their function and doctors could diagnose diseases such as cancer by direct observation.
Yet, when light propagates through most biological tissues, refractive index inhomogeneities cause diffuse scattering that increases with depth. This poses a major challenge to optical techniques, fundamentally limiting their biomedical usefulness to thin sections or cultured cells in vitro and superficial layers of tissue in vivo. As a result, despite many breakthroughs enabled by advances in optical imaging and optogenetics, these techniques are still severely handicapped by scattering.
The goal of our research is to address this challenge by developing new optical techniques based on wavefront engineering and optical time reversal. These approaches, in combination with calcium imaging and electrophysiology, will enable us to study circuits of the brain that have thus far been inaccessible to noninvasive optical methods.
Papadopoulos IN, Jouhanneau JS, Poulet JFA, Judkewitz B. Scattering compensation by focus scanning holographic aberration probing (F-SHARP), Nature Photonics 2017, 11:116-23 doi:10.1038/nphoton.2016.252
Judkewitz B, Horstmeyer R, Vellekoop IM, Papadopoulos IN, Yang C. Translation correlations in anisotropically scattering media, Nature Physics 2015, doi:10.1038/nphys3373; corresponding author: B.J. ; covered in a Nature Physics News & Views article
Judkewitz B *, Wang YM *, Horstmeyer R, Mathy A & Yang C. Speckle-scale focusing in the diffusive regime with time-reversal of variance-encoded light (TROVE), Nature Photonics 2013, 7(4): 300-305; *first & corresponding author; covered in a Nature Photonics News & Views article
Wang YM *, Judkewitz B *, DiMarzio CA, & Yang C. Deep-tissue focal fluorescence imaging with digitally time-reversed ultrasound-encoded light. Nature Communications 2012, 3:928; *first & corresponding author
Wiechert MT, Judkewitz B, Riecke H, Friedrich RW, Mechanisms of pattern decorrelation by recurrent neuronal circuits, Nature Neuroscience 2010, 13 (8): 1003-1010
Judkewitz B, Rizzi M, Kitamura K, Häusser M, Targeted single-cell electroporation of mammalian neurons in vivo, Nature Protocols 2009, 4 (6): 862-869
*Kitamura K, *Judkewitz B, Kano M, Denk W, Häusser M, Targeted patch-clamp recordings and single-cell electroporation of unlabeled neurons in vivo, Nature Methods 2008, 5(1): 61-67; *shared first authorship
Porter J, Craven B, Khan RM, Chang SJ, Kang I, Judkewitz B, Volpe J, Settles G, Sobel N, Mechanisms of scent-tracking in humans, Nature Neuroscience 2007, 10 (1): 27-29
Yaksi E, Judkewitz B, Friedrich RW, Topological reorganization of odor representations in the olfactory bulb, PLoS Biology 2007, 5 (7): e178
Judkewitz B, Roth A, Häusser M, Dendritic enlightenment: using patterned two-photon uncaging to reveal the secrets of the brain's smallest dendrites (editorial/review), Neuron 2006, 50 (2): 180-183
Judkewitz, B. Analysis of functional connectivity in the zebrafish olfactory bulb. Diploma Thesis (2005) supervised by Rainer Friedrich, Max Planck Institute for Medical Research, University of Heidelberg
(Image: Simulated propagation of coherent light through brain tissue)
Our lab is based on the Charité and Humboldt University campus in Berlin Mitte. Located in the middle of town, we are within short walking distance from the central train station and easily reached by numerous public transport lines.
From Tegel Airport (TXL), the TXL bus will take you to our campus in 25 min (get off at Charité station). From Schönefeld Airport (SXF), take the AirPort Express train to Berlin Hauptbahnhof (30 min ride and < 10 min walk to our lab).
Our internal/campus address is Hufelandweg 14 (level 3, room 005). The building is marked by a blue 14 on this campus map. Alternatively, search Google Maps for "Hufelandweg 14, Berlin" or use the embedded map below.